DKW/ Juglans Medium
DKW medium has been defined for in vitro propagation of Paradox Walnut Rootstock (Juglans hindsii x
J. regia) via nodal explants. The explants were placed on medium without
growth-regulators for one week and subsequentely on medium containing 6-BAP and
IBA. Opti-mum shoot development was supported under 4.5 μM 6-BAP and 5 nM IBA.
The basal ends of the tissue culture derived shoots were dipped in 5 mM IBA
solution and subsequen-tly rooted within 10 to 14 days in the greenhouse.
貨号：D0247 DKW/Juglans
Medium含維生(shēng)素
Total
concentration Micro and Macro elements including vitamins: 5584.50 mg/l.
貨号：D0246 DKW/Juglans
Medium不含維生(shēng)素
Total
concentration Micro and Macro elements not including vitamins: 5479.50 mg/l.
|
Macro Elements
|
mg/l
|
mM
|
|
CaCl2
|
112.5
|
1.01
|
|
Ca(NO3)2·2H2O
|
1664.64
|
8.30
|
|
KH2PO4
|
265.00
|
1.95
|
|
K2SO4
|
1559.00
|
8.95
|
|
MgSO4
|
361.49
|
3.00
|
|
NH4NO3
|
1416.00
|
17.70
|
|
Micro Elements
|
mg/l
|
μM
|
|
CuSO4.5H2O
|
0.25
|
1.00
|
|
FeNaEDTA
|
44.63
|
121.61
|
|
H3BO3
|
4.80
|
77.63
|
|
MnSO4.H2O
|
33.80
|
200.00
|
|
Na2MnO4.2H2O
|
0.39
|
1.61
|
|
ZnSO4.7H2O
|
17.00
|
72.19
|
|
Vitamins
|
mg/l
|
μM
|
|
Glycine
|
2.00
|
26.64
|
|
myo-Inositol
|
100.00
|
554.94
|
|
Nicotinic acid
|
1.00
|
8.12
|
|
Thiamine HCl
|
2.00
|
5.93
|