Nitsch Medium
The medium defined by Nitsch was used in the production
of haploid plants of various species of Nicotiana raised from pollen grains. In
this procedure, pollen that were still uninucleate were isolated and then
cultured in vitro. Some pollen grains proliferate into embryo-like structures
that develop in stages similar to those of zygotic embryos. The plantlets
matured and flowered profusely, but did not set seed.
貨号：N0224 Nitsch
Medium含維生(shēng)素
Total
concentration Micro and Macro elements including vitamins: 2178.74 mg/l.
貨号：N0223 Nitsch
Medium不含維生(shēng)素
Total
concentration Micro and Macro elements not including vitamins: 2070.19 mg/l.
|
Macro Elements
|
mg/l
|
mM
|
|
CaCl2
|
166.00
|
1.50
|
|
KH2PO4
|
68.00
|
0.50
|
|
KNO3
|
950.00
|
9.40
|
|
MgSO4
|
90.27
|
0.75
|
|
NH4NO3
|
720.00
|
9.00
|
|
Micro Elements
|
mg/l
|
μM
|
|
CuSO4.5H2O
|
0.025
|
0.10
|
|
FeNaEDTA
|
36.70
|
100.00
|
|
H3BO3
|
10.00
|
161.73
|
|
MnSO4.H2O
|
18.94
|
111.94
|
|
Na2MnO4.2H2O
|
0.25
|
1.03
|
|
ZnSO4.7H2O
|
10.00
|
34.78
|
|
Vitamins
|
mg/l
|
μM
|
|
Biotin
|
0.05
|
0.21
|
|
Folic acid
|
0.50
|
1.13
|
|
Glycine
|
2.00
|
26.64
|
|
myo-Inositol
|
100.00
|
554.94
|
|
Nicotinic acid
|
5.00
|
40.62
|
|
Pyridoxine HCl
|
0.50
|
2.43
|
|
Thiamine HCl
|
0.50
|
1.48
|
Nitsch J.P. and Nitsch C., Haploid plants from
pollen grains, Science 169, 85 (1969). Nitsch J.P., Experimental androgenesis
in Nicotiana, Phytomorphology 19, 389 (1969).