RNaseA Solution 10mg/ml
RNaseA溶液
|
貨号
|
規格
|
價格
|
備注
|
|
|
|
|
|
|
RN001-02
|
10×1ml
|
500元
|
|
|
RN001-03
|
10×300 μl
|
150元
|
配套質粒小提100次用
|
|
RN001-04
|
10×600 μl
|
300元
|
配套質粒小提 200次用
|
|
|
|
|
|
● Introduction：
Ribonuclease A (RNase A) is a single chain polypeptide containing 4
disulfide bridges. In contrast to RNase B it is not a glycoprotein. RNase A can
be inhibited by alkylation of the histidine-12 or histidine-119 which are
present in the active site of the enzyme. Activators of RNase A include
potassium and sodium salts. The optimal temperature for activity is60°C, although the enzyme does exhibit
activity from 15-70°C.
The pH optimum is 7.6, with an activity range of 6-10. The highest activity is exhibited with single
stranded RNA. RNase A is a very stable enzyme and can withstand temperatures up
to100°C. At100°C,
RNase A is most stable between pH 2.0 and 4.5.
● Stability:
RNase A power is recommended to
be stored in a freezer location (-20°C).
Stock solutions stored in frozen aliquots are stable for at least 6 months.
● 質粒提取中RNaseA的使用：
RNaseA在質粒中的終濃度推薦爲100μg/ml，即在質粒溶液中按照(zhào)1/100體(tǐ)積加入10mg/ml RNaseA即可(kě)，如(rú)50μl質粒中加入0.5μl RNaseA溶液，37℃孵育10分(fēn)鍾。