2×Taq PCR MasterMix
● 産品包裝:
|
目錄号
|
包裝
|
價格
|
2×MasterMix(不含染料)
|
RTC3103-02
|
500μl
|
60元
|
RTC3103-03
|
5×500μl
|
240元
|
2×MasterMix(含染料)
|
RTC3104-02
|
500μl
|
60元
|
RTC3104-03
|
5×500μl
|
240元
|
注:以50μl PCR反應體(tǐ)系爲例,每次使用25μl 2×MasterMix,500μl可(kě)做20次 50μl PCR反應。
● 儲存條件(jiàn): -20℃可(kě)長期保存;4℃穩定貯存3個月。經常使用時,一旦融化後請(qǐng)4℃貯存,盡量避免反複凍融。
● 産品簡介:
本産品包含Taq DNA聚合酶、dNTPs、MgCl2、反應緩沖液、PCR反應的增強劑和優化劑以及穩定劑,濃度爲2×。使用時,隻需補加實驗所需的引物和模闆後即可(kě)進行PCR反應,可(kě)最大(dà)限度的減少人(rén)爲誤差,具有快(kuài)速簡便、穩定性好等優點。适用于常規PCR反應、複雜模闆(如(rú)GC含量高,有二級結構)的擴增以及大(dà)規模基因檢測。使用該産品得(de)到的PCR擴增産物末端含有一個A堿基,可(kě)以直接用于TA克隆。
● 質量控制:
經檢測無外源核酸酶活性;PCR方法檢測無宿主殘餘基因組DNA;能有效地擴增人(rén)基因組中的單拷貝基因。
● 使用說(shuō)明:
1. 冰浴中徹底融化2×MasterMix,混勻後Minispin将溶液收集到管底。
2. 按照(zhào)下表在0.2ml PCR管中制備反應體(tǐ)系:
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50μl反應體(tǐ)系
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終濃度
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2×MasterMix
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25μl
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1×
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上遊引物 10μM
|
1-5μl
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0.2-0.8 μM
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下遊引物 10μM
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1-5μl
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0.2-0.8 μM
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模闆
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× μl
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10pg-1μg
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水
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補至50μl
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|
3. 快(kuài)甩離(lí)心将反應液收集到管底。
4. PCR儀如(rú)果沒有熱(rè)蓋加熱(rè)的話(huà),補加25μl礦物油。
5. PCR儀上執行以下程序:
步驟
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溫度
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時間
|
循環數
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初始變性
|
94℃
|
5 min
|
1
|
變性
|
94℃
|
30 sec
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25-40*
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退火(huǒ)
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Tm-5℃*
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30 sec
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延伸
|
72℃
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1 min/kb
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最後延伸
|
72℃
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5 min
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1
|
*注: PCR 反應條件(jiàn)視模闆、引物等的結構條件(jiàn)不同而各異。在實際操作(zuò)中需根據模闆、目的片段的大(dà)小、堿基序列和引物的長短(duǎn)等具體(tǐ)情況,設定最佳的反應條件(jiàn)(溫度、時間等)。
産品示例:
2×Taq PCR MasterMix擴增效果比較
M III:RTM403 DNA Marker III
lane 1:Rice 5000 bp
lane 2:Ricie 3000 bp
lane 3: Rice 600 bp
引用RTC3104 2×Taq PCR MasterMix含染料發表文章(zhāng)
1. [2013 IF=1.63] Preventive effect of
total glycosides from Ligustri Lucidi Fructus against nonalcoholic fatty liver
in mice
Author: Nianyun Yang, Yiwen Zhang and Jianming Guo
Product:RTC3104 2×Taq PCR MasterMix含染料
Journal: Z. Naturforsch. 2015; 70(9-10)c:
237–241
Institution:Nanjing University of Chinese Medicine
Paper link: https://pubmed.ncbi.nlm.nih.gov/26501160/
2. [2015 IF=1.377] Expression and
localization of guanine
nucleotide-binding protein
alpha S in
the testis and
epididymis of rams
at different developmental stages
Author: Zhen Li, Jieli
Lu, Jia Chen,
Quanhai Pang, Ruipeng
Nan, Zhiwei Zhu
Product:RTC3104 2×Taq PCR MasterMix含染料
Journal: Animal
Reproduction Science. Volume
178, March 2017, Pages 31-39
Institution:Shanxi Agricultural University
Paper link:https://www.sciencedirect.com/science/article/pii/S0378432016307187
3. [2018 IF=2.9] Integration of gene
expression profile data to screen and verify immune-related genes of chicken
erythrocytes involved in disease virus Marek’s
Author: Ali Raza Jahejo, Sayyad Ali Raza Bukhari, Fa-Jie Jia,
Sayed Haidar Abbas Raza, Mujahid Ali Shah, Nasir Rajput, Anam Ahsan, Sheng Niu,
Guan-Bao Ning, Ding Zhang, Yu-Hai Bi, Qi-Hui Wang, Ling-Xia Han, Wen-Xia Tian
Product:RTC3104 2×Taq PCR MasterMix含染料
Journal: Microbial Pathogenesis. 148 (2020)
104454
Institution:Shanxi Agricultural University
Paper link:https://doi.org/10.1016/j.micpath.2020.104454
4. [2018 IF=1.69] Study on the correlation
between BMPR1Bprotein in sheep blood and reproductive performance
Author: Xiaoyan Zhang, Liping Zhang, Weibo Sun, Xia Lang,
Jianping Wu, Caiye Zhu,Jianlei Jia, Jinpen Jin, Younfu La, David P. Casper
Product:RTC3104 2×Taq PCR MasterMix含染料
Journal: Journal of Animal Science. 2020 May
1;98(5)
Institution:Gansu Agricultural University
Paper link:https://pubmed.ncbi.nlm.nih.gov/32300800/
5. [2019 IF=2.6] Natural occurrence
of Alternaria mycotoxins in wheat and potential of reducing
associated risks using magnolol.
Author: Dongmei Jiang, DizheWei, Hongtao Li,LiuqingWang,Nan
Jiang,Yunchao Li, Meng Wang
Product:RTC3104 2×Taq PCR MasterMix含染料
Journal: Journal of the Science
of Food and Agriculture. Volume101, Issue7,May 2021
Institution:Beijing Academy of Agriculture and
Forestry Sciences
Paper link: https://doi.org/10.1002/jsfa.10901