1kb plus DNA ladder（300-10000bp）

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1kb plus DNA ladder（300-10000bp）

産品編号：RTM418-02

産品規格：100次

數量

價格￥200

精準定量1kb plus DNA Ladder

● 産品編号及規格：

RTM418-02 100T(2×250μl)

本産品是由12條帶狀雙鏈DNA條帶組成，适用于瓊脂糖凝膠電泳中DNA條帶的分(fēn)析。

本産品爲即用型産品，已含有1×loading buffer，可(kě)根據實驗需要，直接取2-5 μl電泳，使用方便，電泳圖像清晰。本産品中12條帶分(fēn)爲300（50 ng/5 μl），500（50 ng/5 μl），800（50 ng/5 μl），1000（50 ng/5 μl），1500（50 ng/5 μl），2000（50 ng/5 μl），3000（50 ng/5 μl），4000（100 ng/5 μl），5000（50 ng/5 μl），6000（50 ng/5 μl），8000（50 ng/5 μl），10000bp（50 ng/5 μl）。其中2000bp條帶加亮顯示。

● 儲存及運輸：

4℃貯存3個月， -20℃長期貯存；常溫運輸。

● 使用方法：

1. 取2-5μl本産品加入到瓊脂糖凝膠的加樣孔中（每1mm×1mm加樣孔加1μl，如(rú)果加樣孔寬于5mm，可(kě)以适當增加上樣量）就(jiù)行電泳。

2. 建議(yì)電泳條件(jiàn):凝膠濃度爲0.8-1.0％，凝膠長度10 cm，電泳電壓~7v/cm，電泳時間60+分(fēn)鍾。

3. 通過核酸染料染色後紫外燈或藍光(guāng)儀下觀察條帶。

● 注意事(shì)項：

1. 瓊脂糖的質量對DNA的電泳有很大(dà)影(yǐng)響，電泳時請(qǐng)盡量使用質量優等的瓊脂糖。由于PAGE電泳的特殊性，該産品不建議(yì)用于PAGE電泳。

2. 使用與電泳緩沖液一緻的緩沖液融化瓊脂糖，例如(rú)使用1×TAE緩沖液電泳，需使用1×TAE緩沖液溶膠。

3. 多次電泳後緩沖液電導增強，相(xiàng)同電壓下電流增大(dà)，導緻分(fēn)辨率降低，并且明顯産熱(rè)，嚴重時會引起凝膠熔化或DNA變性。

4. 推薦使用RealSafe系列染料（貨号：GR002，GR003，GR004）染色。如(rú)果使用Goldview或EB等染料進行染色，由于此類染料帶更多的正電荷（染料移動方向與核酸泳動方向相(xiàng)反），随着電泳時間延長，染料向大(dà)片段聚集，凝膠會出現陰陽膠現象（染料含量高的凝膠紫外下較亮，含量低的凝膠紫外下發暗），導緻小分(fēn)子量DNA顯色很弱或不可(kě)見(jiàn)。陰陽膠可(kě)以在電泳結束後浸泡染色，将膠浸沒在含1 μg /ml EB或Goldview的電泳緩沖液中20-25 min，小分(fēn)子量DNA會顯色清楚。更長時間浸泡會因DNA分(fēn)子擴散而使條帶彌散，小分(fēn)子量DNA更易彌散。

5. 紫外照(zhào)射下EB易分(fēn)解，使DNA條帶熒光(guāng)變淺。DNA受紫外光(guāng)照(zhào)射形成嘧啶二聚體(tǐ)，不利于待回收片段的下遊工(gōng)作(zuò)，因此盡可(kě)能減少紫外照(zhào)射時間。

6. 蔗糖會結合DNA，降低其電泳遷移率，建議(yì)DNA樣品上樣使用含甘油的Loading Buffer。

7. 大(dà)部分(fēn)核酸工(gōng)具酶會結合DNA，降低其電泳遷移率，大(dà)分(fēn)子量DNA尤爲明顯。

● 實驗示例：

● 附錄：

DNA在瓊脂糖凝膠中的有效分(fēn)離(lí)範圍

瓊脂糖濃度%（w/v）	雙鏈DNA有效分(fēn)離(lí)範圍（bp）	優選電泳緩沖液	示蹤染料相(xiàng)當于雙鏈DNA片段粗略大(dà)小(bp)
			溴酚藍		二甲苯菁
			TBE	TAE	TBE	TAE
0.5	2000-50000	1×TAE	750	1150	13000	16700
0.8	800-10000	1×TAE	320	530	4830	6500
1.0	400-8000	1×TAE	220	370	3030	4160
1.2	300-7000	1×TAE/0.5×TBE	160	275	2070	2890
1.5	200-3000	1×TAE/0.5×TBE	110	190	1300	1840
2.0	100-2000	0.5×TBE	65	120	710	1040
3.0	25-1000	0.5×TBE	30	60	300	460

使用DNA Marker産品發表部分(fēn)文章(zhāng)列表：

1. [2021 IF=1.17] Dendrostoma covidicola sp. nov. (Erythrogloeaceae, Diaporthales) on Fagus sylvatica from Sichuan Province, China.

Author: MILAN C. SAMARAKOON, ITTHAYAKORN PROMPUTTHA & JIAN-KUI (JACK) LI

Marker: RTM415，D2000 DNA ladder

Journal: Phytotaxa. 2021，483 (2): 085–094.

Institution：University of Electronic Science and Technology of China

Paper link：https://doi.org/10.11646/phytotaxa.483.2.1

2.[2021 IF=6.057] MUC1 detection and in situ imaging method based on aptamer conformational switch and hybridization chain reaction.

Marker :RTM403，DNA Marker III

Author: ZihanSong，ShengCai.

Journal: Talanta. Vol 239, 2022, 123129

Institution： Zhejiang University

Paper link：https://doi.org/10.1016/j.ijbiomac.2021.11.194

3. [2021 IF=3.24] Curing of Plasmid pXO1 from Bacillus anthracis Using Plasmid Incompatibility.

Marker :RTM404，DNA Marker IV

Author: Xiankai Liu, Dongshu Wang, Huagui Wang, Erling Feng, Li Zhu, Hengliang Wang

Journal: PLos one，January 2012 | Volume 7 | Issue 1 | e29875

Institution：State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology

Paper link： https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0029875

4. [2021 IF=3.24] Identification of a Novel Fungus, Leptosphaerulina chartarum SJTU59 and Characterization of Its Xylanolytic Enzymes.

Marker :RTM409，100bp DNA ladder

Author: Qiong Wu, Yaqian Li, Yingying Li, Shigang Gao, Meng Wang, Tailong Zhang, Jie Chen

Journal: PLos one，September 2013 | Volume 8 | Issue 9 | e737

Institution：School of Agriculture and Biology, Shanghai Jiao Tong University

Paper link：https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0073729

5. [2021 IF=3.08] Low genetic diversity and local adaptive divergence of Dracaena cambodiana (Liliaceae) populations associated with historical population bottlenecks and natural selection: an endangered long-lived tree endemic to Hainan Island, China.

Marker :RTM409，100bp DNA ladder

Author: D.-J. Zheng, L.-S. Xie, J.-H. Zhu，Z.-L. Zhang

Journal: Plant Biology，14 (2012) 828–838

Institution：Key Laboratory of Crop Genetics and Breeding of Hainan Province, Hainan Academy of Agricultural Sciences

Paper link：https://onlinelibrary.wiley.com/doi/10.1111/j.1438-8677.2011.00559.x

6. [2021 IF=3.24] Envelope Proteins ofWhite Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT).

Marker :RTM415，D2000 DNA ladder

Author: Shijun Xie, Xiaojun Zhang, Jiquan Zhang, Fuhua Li, Jianhai Xiang

Journal: Plos One，Published: December 21, 2015

Institution：Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences

Paper link： https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0144922

7. [2021 IF=1.17] Rhopalostroma brevistipitatum sp. nov. from Thailand with an extended generic description for Rhopalostroma.

Marker :RTM415，D2000 DNA ladder

Author: DINUSHANI A. DARANAGAMA, XINGZHONG LIU, SUNITA CHAMYUANG, MARC STADLER，ALI H.BAHKALI, KEVIN D. HYDE

Journal: Phytotaxa，2015，227 (3): 229–242

Institution：State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences

Paper link：https://www.biotaxa.org/Phytotaxa/article/view/phytotaxa.227.3.2/0

8. [2021 IF=20.37] A multi-locus phylogenetic evaluation of Diaporthe (Phomopsis)

Marker :RTM415，D2000 DNA ladder

Author: Dhanushka Udayanga & Xingzhong Liu &Pedro W. Crous & Eric H. C. McKenzie &Ekachai Chukeatirote & Kevin D. Hyde

Journal: Fungal Diversity (2012) 56:157–171

Institution：State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences

Paper link：https://link.springer.com/article/10.1007/s13225-012-0190-9

9. [2021 IF=2.9] An easily-performed high-throughput method for plant genomic DNA extraction.

Marker :RTM421，λ DNA/HindIII

Author: Deshui Yu, Ju Zhang, Guangxuan Tan, Ningshu Yu, Qiuyue Wang, Qiqi Duan, Xin

Qi, Mingjiao Cheng, Chunxue Yan, Zhangkun Wei, Zhenmiao Yu, Wenchao Huang, Chengwei Li

Journal: Analytical Biochemistry Volume 569, 15 March 2019, Pages 28-30

Institution：Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal

University

Paper link：

https://www.sciencedirect.com/science/article/abs/pii/S0003269718310637?via%3Dihub

10. [2021 IF=2.88] Genetic diversity and genetic structure of Decalobanthus boisianus in Hainan Island, China.

Marker :RTM415，D2000 DNA ladder

Author: Huan Jiang，Wenxing Long， Hui Zhang，Chengneng Mi，Tao Zhou，Zongzhu Chen

Journal: Ecology and Evolotion Volume 569, 15 March 2019, Pages 28-30

Institution：Institute of Tropical Agriculture and Forestry, Hainan University

Paper link： https://doi.org/10.1002/ece3.5127

11. [2021 IF=5.64] Identi?cation of a Novel Hypovirulence-Inducing Hypovirus From Alternaria alternata.

Marker :RTM406，DNA Marker VI

Author: Huan Li, Ruiling Bian, Qian Liu, Liu Yang, Tianxing Pang, Lakha Salaipeth,

Ida Bagus Andika, Hideki Kondo and Liying Sun

Journal: Frontiers in Microbiology May 2019 | Volume 10 | Article 1076

Institution：State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University

Paper link： https://www.frontiersin.org/articles/10.3389/fmicb.2019.01076/full

12. [2021 IF=2.6] Natural occurrence of Alternaria mycotoxins in wheat and potential of reducing associated risks using magnolol.

Marker :RTM415，D2000 DNA ladder

Author: Dongmei Jiang, Dizhe Wei, Hongtao Li, Liuqing Wang, Nan Jiang,Yunchao Li and Meng Wang

Journal: Journal of the Science of Food and Agriculture Volume101, Issue7，2021，3071-3077

Institution：Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences

Paper link：https://onlinelibrary.wiley.com/doi/10.1002/jsfa.10901

13. [2021 IF=20.37] Taxonomy, Phylogeny, Molecular Dating and Ancestral State Reconstruction of Xylariomycetidae (Sordariomycetes)

Marker :RTM415，D2000 DNA ladder

Author: Kevin D Hyde，Jian-Kui Liu

Journal: Fungal Diversity volume 112, pages 1–88 (2022)

Institution：School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China

Paper link：https://link.springer.com/article/10.1007/s13225-021-00495-5

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