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RNAplant植物RNA提取試劑(多糖多酚植物)

RNAplant植物RNA提取試劑(多糖多酚植物)

産品編号:RTR2303

産品規格:100ml

數量
價格 ¥500

RNAplant

植物RNA提取試劑


産品簡介:

RNAplant試劑可(kě)從(cóng)植物組織,特别是富含多酚或澱粉的植物組織中(如(rú)馬鈴薯塊莖,白(bái)松松針或嫩苗,和西紅(hóng)柿葉等)提取高純度的總RNA

100 ml可(kě)處理(lǐ)100 mg組織200次。

保存條件(jiàn):室溫運輸,4保存,可(kě)保存6-12個月。 

準備試劑液氮,研缽,無RNase離(lí)心管,5 M NaClNC1050F 5M 氯化鈉 RNase-free,DNase-free),氯仿,異丙醇,75%乙醇(無RNase水配制),無RNase水。


小規模提取操作(zuò)步驟(樣品<0.1 g ,使用小規模提取方法):

1.      取不多于0.1 g植物組織,在液氮中研碎,轉移到1.5 ml 離(lí)心管中,加0.5 ml提取試劑,振蕩至徹底混勻。

注:振蕩不要太劇(jù)烈,否則容易導緻基因組DNA污染。

2.      室溫放(fàng)置5分(fēn)鍾。

3.      4℃ 12, 000 rpm離(lí)心2-3分(fēn)鍾,上清轉入新的無RNase離(lí)心管。

4.      上清中加入0.1 ml 5 M NaClNC1050F 5M 氯化鈉 RNase-free,DNase-free),溫和混勻。

5.      加入0.3 ml 氯仿,上下颠倒混勻。

6.      4℃ 12,000 rpm離(lí)心10-15分(fēn)鍾,取上層水相(xiàng)轉入新的無RNase離(lí)心管。

7.      加與所得(de)水相(xiàng)等體(tǐ)積的異丙醇,混勻,室溫放(fàng)置10分(fēn)鍾。

8.      4℃ 12,000 rpm離(lí)心10-15分(fēn)鍾。棄掉上清,注意不要倒出沉澱。加1 ml 75%乙醇沖洗沉澱。(沉澱可(kě)能很難看(kàn)見(jiàn),應小心操作(zuò)。)

9.      4℃ 5000-7000rpm離(lí)心3分(fēn)鍾。倒出液體(tǐ),注意不要倒出沉澱。剩餘的少量液體(tǐ)短(duǎn)暫離(lí)心,用槍頭吸出,室溫晾幹1-2分(fēn)鍾至沉澱幹燥。

10.  加适量無RNase水充分(fēn)溶解RNA-70℃保存。

注意事(shì)項:

RNAplant有刺激性味道,操作(zuò)時請(qǐng)在通風(fēng)處進行。

RTR2303 RNAplant 發表文章(zhāng)列表:

 

1. [2021 IF=4.7] The 5-untranslated region of the FAD3 mRNA is required for its translational enhancement at  low temperature in Arabidopsis roots.

Author: Chun-TaoWang, Yi-Nong Xu.

Journal: Plant Science 179,234-240,2010

植物材料:拟南(nán)芥

Institution: Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences,

Paper linkhttps://linkinghub.elsevier.com/retrieve/pii/S0168945210001470

 

2. [2021 IF=4.1] Different exogenous sugars affect the hormone signal pathway and sugar metabolism in ‘‘Red  Globe’’ (Vitis vinifera L.) plantlets grown in vitro as shown by transcriptomic analysis.

Author: Juan Mao,Wenfang Li,Baoqin Mi,Zonghuan Ma,Yongmei Zhang, Baihong Chen

Journal: Planta 246, 537–552,2017

植物材料:葡萄

Institution: College of Horticulture, Gansu Agricultural University

Paper linkhttps://link.springer.com/article/10.1007/s00425-017-2712-x

 

3. [2021 IF=3.4] Transcriptome analysis revealed glucose application afects plant hormone signal transduction  pathway in “Red Globe” grape plantlets s.

Author: Juan Mao ,Wenfang Li, Baoqin Mi, Zonghuan Ma,  Mohammed Mujitaba Dawuda, Cunwu Zuo

Journal: Plant Growth Regul volume 84, pages 45–56 (2018)

植物材料:葡萄

Institution: College of Horticulture, Gansu Agricultural University

Paper linkhttps://link.springer.com/article/10.1007/s10725-017-0320-1

 

4. [2021 IF=4.169] Effects of CEPA and 1-MCP on flower bud differentiation of apple cv.  1 agafu No.2 grafted on different rootstocks.

Author: Wenfang Li, Baihong Chen

Journal: J Plant Growth Regul. volume 38, pages 842–854 (2019)

植物材料:蘋果

Institution: College of Horticulture, Gansu Agricultural University

Paper linkhttps://link.springer.com/article/10.1007/s00344-018-9895-7

 

5. [2021 IF=7.228] FIT and bHLH Ib transcription factors modulate iron and copper crosstalk in Arabidopsis.

Author: Yuerong Cai, Yang Li, Gang Liang

Journal: Plant, Cell and Environment. Volume44, Issue5 May 2021 Pages 1679-1691

植物材料:拟南(nán)芥

Institution: CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden

Paper linkhttps://onlinelibrary.wiley.com/doi/10.1111/pce.14000

 

6. [2022 IF=6.2] Genome-Wide Identification and Abiotic Stress Response Analysis of PP2C Gene Family in Woodland and Pineapple Strawberries.

Author: Lili Guo, Shixiong Lu, Tao Liu, Guojie Nai, Jiaxuan Ren, Huimin Gou, Baihong Chen and Juan Mao

Journal: International Journal of Molecular Sciences. 2023, 24, 4049.

植物材料:草莓

Institution: College of Horticulture, Gansu Agricultural University

Paper linkhttps://doi.org/10.3390/ijms24044049

 

7. [2022 IF=2.561] Physiological and Transcriptomic Analysis Reveals Drought?Stress Responses of Arabidopsis DREB1A in Transgenic Potato

Author: Xiaoxia Jia, Enfang Qi, Shi Liu, Sheng Ma, Guohong Wen, Xinhui Zhang,  Heping Lv, Wei Huang, Xucheng Zhang

Journal: Potato Research Published online 21 Feb 2023

植物材料:馬鈴薯

Institution: Gansu Academy of Agricultural Sciences

Paper linkhttps://link.springer.com/article/10.1007/s11540-023-09619-7

 

8. [2022 IF=6.6] Molecular evolution and expression assessment of DFRs in apple

Author: Wen?Fang Li, Ju Gao, Zong?Huan Ma, Ying?Jun Hou, Xin Li, Juan Mao and Bai?Hong Chen

Journal: Chemical and Biological Technologies in Agriculture (2023) 10:98

植物材料:蘋果

Institution: College of Horticulture, Gansu Agricultural University

Paper linkhttps://doi.org/10.1186/s40538-023-00470-z

 

9. [2022 IF=3.5] Genome-wide identification and expression analysis of the BURP domain-containing genes in Gossypium hirsutum

Author: Jiaxuan Ren,Li Feng,Lili Guo, Huimin Gou,Shixiong Lu, Juan Mao

Journal: Physiology and Molecular Biology of Plants 2023 29(11):1717–1731

植物材料:棉花

Institution: College of Horticulture, Gansu Agricultural University

Paper linkhttps://doi.org/10.1007/s12298-023-01393-7

 


 
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